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IMAGE STACKS AND AUTOMONTAGE RESULTS Prof. John Hart Program in Atmospheric and Oceanic Sciences University of Colorado Boulder, CO 80302 November 2003 nimbus.colorado.edu/hart/science.htm This page contains links to jpeg images that are Q7 (medium) compression files rendered in Photoshop from original 1600x1200 bitmaps. These original bitmaps were obtained by a) Reducing 5MP images, shot through a microscope using a Nikon CP5000 digital camera, from ~2500x2000 to 1600x1200 in one step via Photoshop (bicubic resampling). b) Processing stacks of the reduced-size bitmap files in the Syncroscopy program AutoMontage. There are three image stacks: 1) A reflected light microscope image of a hole in a leaf: 4X APO objective, 24 layers in the stack (labeled LH46 to LH69). This image stack and the processing of it is described at length in my writeup on Extended DOF Processing. This image is called LH . 2) A reflected light macro-photo stack of a yellow flower, obtained by step-focusing a Nikon CP5000 digital camera in its macro mode. There are only 4 layers (labelled CP37 to CP40). This image and its processing was also discussed in the ExDOF paper. It can be viewed in various 3D forms. that were obtained by using a slidebar and applying ExDOF processing to both L and R sides independently. In the list below is also a parallel pair derived from AutoMontage using a single stack to give a stereo L-R parallel pair. 3) An orange lily interior, also obtained by step-focusing a Nikon CP5000 over 6 layers (Lily01 to Lily 06). This can be viewed in various 3D forms obtained by slidebar. In the list below is also a parallel pair derived from AutoMontage using a single stack. Depth Maps (DM): Depth maps are given for each of the three image stacks. In AutoMontage, an integer depth map (with values corresponding to the "in-focus" plane of the stack, e.g. a value 35 for file CP35), is only directly obtained for the "Fixed" method of Scanning the stack. In all other Scan Methods (Blended, Weighted, Compound-Weighted, Exponentially-Weighted), a floating point Depth Map is generated. When the latter is saved to disk it can be truncated back to integer form with the same representation as one would obtain using the Fixed Method (i.e. having as many integers as the original stack has layers). Such depth-map files have _I appended to the file-name. The full floating point depth maps (that reflect complicated interpolations in the scanning algorithms), are converted to 255 levels and appended with _F. The most accurate AutoMontage scanning method for 3D reconstruction is Blended. Certain subjects (like the yellow flower), which have big jumps in focus point (petal edge to background, for example) do better with various weighted methods (see the Technote). Montage (Mon): The composite "infocus" image of the stack. ParPair (PP): Parallel pair constructed by AutoMontage from the single stack whose data are listed. Note. When there are very few layers (as in Yellow Flower), AutoMontage has a difficult time producing a realy good PP (or at least I haven't yet figured out how to make it do so). The PP for yellow flower is not as good as the slidebar version. Note on Step-Focusing: The macro stacks were made using the Nikon CP5000's internal focus in manual control (MF) mode. Unfortunately this only allows manual stepping in pre-set intervals of distance inherent to the camera (which cannot be changed). Some of the steps were a bit larger than I would have liked. But a test is just a test..... Files (all 1600x1200 except PP, which are 3200x1200): Leaf Hole: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, DM1, DM2, Mon, PP Yellow Flower: 1 , 2 , 3 , 4 , DM1 , DM2 , Mon , PP Lily: 1 , 2 , 3 , 4 , 5 , 6 , DM , Mon , PP , SlidebarPP
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